48-660MAG
MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
Synonym(s):
Cell Signaling Kit, MAPK/SAPK 10-Plex Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Recommended Products
species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
assay range
inter-assay cv: 4-13%
intra-assay cv: 3-10%
detection method
fluorometric (Luminex xMAP)
storage temp.
2-8°C
General description
Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- An overnight (4°C) incubation is recommended for best results.
- This assay requires 25 μL diluted cell lysate.
- This kit must be run using Assay Buffer 2 (provided).
- 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Research Category
Signaling
Signaling
Used to detect/quantify:ATF2 (Thr71)|Erk (Thr185/Tyr187)|HSP27 (Ser78)|JNK (Thr183/Tyr185)|c-Jun (Ser73)|MEK1 (Ser222)|MSK1 (Ser212)|p38 (Thr180/Tyr182)|p53 (Ser15)|STAT1 (Tyr701)
Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Cheng-Wei Wu et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 246-247, 110461-110461 (2020-06-05)
In arid conditions, the African Clawed frog Xenopus laevis enters a state of estivation dormancy as an adaptive survival strategy. Under estivation, X. laevis experience severe dehydration stress as 25-35% of total body water is lost. Dehydration in X. laevis
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