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Key Documents

05-1098

Sigma-Aldrich

Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2

clone 7D2.2, from mouse

Synonym(s):

acetyl-CoA carboxylase 1, acetyl-CoA carboxylase-alpha, acetyl-Coenzyme A carboxylase alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7D2.2, monoclonal

species reactivity

human, rat

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACACA(31)
rat ... Acaca(60581)

General description

Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Immunogen

Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.

Application

Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.

Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.

Quality

Evaluated by Western Blot in human heart lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.

Target description

~ 266 kDa Observed

Linkage

Replaces: 04-322

Physical form

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human heart lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Samanta Kviklyte et al.
American journal of physiology. Endocrinology and metabolism, 313(1), E48-E62 (2017-03-23)
AMP-activated protein kinase (AMPK) plays a key role in energy homeostasis and is activated in response to contraction-induced ATP depletion in skeletal muscle via a rise in intracellular AMP/ADP concentrations. AMP can be deaminated by AMP-deaminase (AMPD) to IMP, which
Ana M Cruz et al.
Frontiers in endocrinology, 12, 697445-697445 (2022-01-04)
We evaluated the efficacy of a novel brain permeable "metformin-like" AMP-activated protein kinase activator, R481, in regulating glucose homeostasis. We used glucose sensing hypothalamic GT1-7 neuronal cells and pancreatic αTC1.9 α-cells to examine the effect of R481 on AMPK pathway
Nicole K H Yiew et al.
iScience, 26(11), 108196-108196 (2023-11-09)
The liver coordinates the systemic response to nutrient deprivation and availability by producing glucose from gluconeogenesis during fasting and synthesizing lipids via de novo lipogenesis (DNL) when carbohydrates are abundant. Mitochondrial pyruvate metabolism is thought to play important roles in
Dandan Zhong et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(3), 1163-1179 (2018-09-10)
Non-alcoholic fatty liver disease (NAFLD) encompasses a series of pathologic changes ranging from steatosis to steatohepatitis, which may progress to cirrhosis and hepatocellular carcinoma. The purpose of this study was to determine whether ganoderma lucidum polysaccharide peptide (GLPP) has therapeutic
Sebastian Trousil et al.
Oncotarget, 7(24), 37103-37120 (2016-05-22)
The glycerophospholipid phosphatidylcholine is the most abundant phospholipid species of eukaryotic membranes and essential for structural integrity and signaling function of cell membranes required for cancer cell growth. Inhibition of choline kinase alpha (CHKA), the first committed step to phosphatidylcholine

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