Multiplexing Tips & Tricks

We’re so confident in the benefits of MILLIPLEX^® multiplex kits that we’ve compiled tips and tricks, straight from the experts, to eliminate any doubt in your ability to multiplex like a pro. Enhance the power of your research with MILLIPLEX^® multiplexing by learning more about industry guidance, multiplexing tips, one-day multiplex assays for quick turnaround times, and more.

• Industry Guidance for Immunoassays
• Multiplexing Tips and Tricks
• One-Day MILLIPLEX^® Multiplex Assays for Limited Lab Time
• Request Information

Industry Guidance for Immunoassays

Let industry guidance lead you to MILLIPLEX^® multiplexing. From academia to contract research to pharma, MILLIPLEX^® kits meet the ever-increasing demand for high-quality immunoassays for reproducible results. This includes:

• Detection and sensitivity
• Performance in a sample matrix
• Specificity
• Selectivity
• Precision and accuracy
• Linearity
• Stability
• Cross-talk
• Lot-to-lot reproducibility
• Vendor support

Want to learn more about industry guidance on assay development and validation? We recommend these references^1,2,3 below.

Multiplexing Tips and Tricks

Check out these articles for tips and tricks on multiplexing with MILLIPLEX^® assays.

Choosing the Right Assay, Platform, and Materials

• Decide which MILLIPLEX^® assay is right for your research and the materials required
• Choose the best Luminex^® multiplex platform for your research
• Species cross-reactivity of MILLIPLEX^® multiplex assays

Preparation Tips

• Sample preparation techniques
• Preparation of reagents
• Using MILLIPLEX^® kits in alternate sample types

Running Assays and Analyzing Data

• Immunoassay procedures and troubleshooting tips
• Equipment settings and data analysis

General Questions on MILLIPLEX^® Assays

• MILLIPLEX^® Multiplex Assays FAQs

One-Day MILLIPLEX^® Multiplex Assays for Limited Lab Time

Many of our MILLIPLEX^® multiplex assays can be completed in a single day by using a shorter primary incubation time. With only 90 minutes of hands-on time required⁴, these assays allow for more time to work on other important laboratory projects. Results are available in 4-5 hours, depending on incubation times, making them ideal for limited laboratory time.

The typical MILLIPLEX^® one-day assay workflow is as follows:

• Step 1: Set up plate with samples/standards, beads, buffer/matrix. Incubate at room temperature for 1-2 hours*.
• Step 2: Wash plate. Add detection antibodies. Incubate at room temperature for 1 hour*.
• Step 3: Wash plate*. Add Streptavidin-Phycoerythrin. Incubate at room temperature for 30 minutes.
• Step 4: Wash plate. Read on Luminex^® instrument.

*See protocol for specific incubation times and wash steps.

More than 50 MILLIPLEX^® multiplex kits, covering several research areas, are available with a single-day protocol. This includes more than 30 immunology assays, as well as virology, metabolism/endocrinology, cardiovascular, neuroscience, and more. MILLIPLEX^® multiplex assays spanning multiple species can be completed in one day, including human, mouse, rat, non-human primate, and many of our veterinary medicine and animal health panels. Select single-day assays are included below.