Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp).

Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp).

Journal of pharmaceutical and biomedical analysis (2017-06-14)

Virginia Brighenti, Federica Pellati, Marleen Steinbach, Davide Maran, Stefania Benvenuti

RÉSUMÉ

The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed to identify samples with a high content of bioactive compounds, with a view to underscoring the importance of quality control in derived products as well. Different extraction methods, including dynamic maceration (DM), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical-fluid extraction (SFE) were applied and compared in order to obtain a high yield of the target analytes from hemp. Dynamic maceration for 45min with ethanol (EtOH) at room temperature proved to be the most suitable technique for the extraction of cannabinoids in hemp samples. The analysis of the target analytes in hemp extracts was carried out by developing a new reversed-phase high-performance liquid chromatography (HPLC) method coupled with diode array (UV/DAD) and electrospray ionization-mass spectrometry (ESI-MS) detection, by using an ion trap mass analyser. An Ascentis Express C

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 3 mm

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Ascentis^® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

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Ascentis^® Express C18, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

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Ascentis^® Express C18, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 2.1 mm

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Ascentis^® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 3 cm × 2.1 mm

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Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 7.5 cm × 2.1 mm

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Ascentis^® Express C18, 2 μm Guard Cartridge, 2 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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Ascentis^® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm

50536-U

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Ascentis^® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

50462-U

Supelco

Ascentis^® Express C18 PCP HPLC Columns, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

53811-U

Supelco

Colonne de CLHP Ascentis^® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 3 mm

50542-U

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Ascentis^® Express C18, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

50509-U

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Ascentis^® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

50819-U

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Ascentis^® Express C18, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 3 mm