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  • Metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the European sea bass (Dicentrarchus labrax).

Metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the European sea bass (Dicentrarchus labrax).

Environmental pollution (Barking, Essex : 1987) (2013-01-01)
Denise Fernandes, Giorgio Dimastrogiovanni, Mercedes Blázquez, Cinta Porte
RÉSUMÉ

This study investigates the metabolism and mode of action of galaxolide (HHCB) in the European sea bass -Dicentrarchus labrax- following a single intraperitoneal injection of 50 mg HHCB/kg body weight. In addition, a group of fish was injected with 50 mg/kg of ketoconazole (KCZ), a fungicide that is known to interfere with different Cyp isoenzymes. HHCB was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. Both, HHCB and a hydroxylated metabolite were detected in bile. The fungicide ketoconazole was a strong inhibitor of Cyp11β and Cyp3a-catalyzed activities. The work contributes to the better understanding of the impact of synthetic musks on fish and proposes the determination of HHCB and/or its hydroxylated metabolite in bile as a tool to assess environmental exposure in wild fish.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
17α-Hydroxyprogesterone, ≥95%
Sigma-Aldrich
Ketoconazole, 99.0-101.0% (EP, titration)
Supelco
Androstenedione solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
17α-Hydroxyprogesterone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
4-Androstene-3,17-dione, VETRANAL®, analytical standard
Ketoconazole, European Pharmacopoeia (EP) Reference Standard