- Caged glucosamine-6-phosphate for the light-control of riboswitch activity.
Caged glucosamine-6-phosphate for the light-control of riboswitch activity.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (2011-10-19)
Bernhard Wulffen, Maximilian Clemens Rudolf Buff, Monika Pofahl, Günter Mayer, Alexander Heckel
PMID22005771
RÉSUMÉ
We have synthesized a light-activatable ("caged") derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3'-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro.