- Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea.
Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea.
Bio-protocol (2017-07-05)
Giovanna Salbitani, Claudia Bottone, Simona Carfagna
PMID34541114
RÉSUMÉ
Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.
MATÉRIAUX
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Marque
Description du produit
Sigma-Aldrich
Acide éthylènediaminetétraacétique disodium salt dihydrate, suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)
Sigma-Aldrich
Acide 5,5′-Dithiobis(2-nitrobenzoïque), ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Sigma-Aldrich
Glutathione Reductase from baker′s yeast (S. cerevisiae), ammonium sulfate suspension, 100-300 units/mg protein (biuret)
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide phosphate, reduced tetra(cyclohexylammonium) salt, ≥93%