SRP6014

HGF human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonyme(s) : F-TCF, HGF, HGFB, Hepatopoietin (HPTA), Scatter Factor (SF)
• Numéro CAS: 953836-17-4
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in CHO cells
• Pureté: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 80.017 kDa
• Conditionnement: pkg of 1 mg; pkg of 10 μg
• Technique(s): cell culture | embryo: suitable
• Impuretés: Endotoxin, tested
• Numéro d'accès NCBI: P14210
• Numéro d'accès UniProt: P14210
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... HGF(3082)

Description

Description générale

Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques.

Actions biochimiques/physiologiques

Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors.

Forme physique

The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Reconstitution

Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm^-1 H₂O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable