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SHPH01

Sigma-Aldrich

MISSION® LentiPlex® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

Synonyme(s) :

Human shRNA Library, LentiPlex shRNA Library

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About This Item

Code UNSPSC :
41105904
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Gamme de produits

MISSION®

Concentration

≥5x108 VP/ml (via p24 assay)

Technique(s)

capture ELISA: 5 × 108 TU/mL using p24

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.

Please visit sigma.com/lentiplex to learn more and see example data and uses.

Application

MISSION® LentiPlex® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis.

Autres remarques

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Informations légales

Lentiviral License Agreement
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Nécessaire, mais non fourni

Réf. du produit
Description
Tarif

M8787

Chlorure de magnésium solution, PCR Reagent, 25 mM MgCI2 solution for PCR

H9268

Hexadimethrine bromide, ≥94% (titration)

D3687

DirectLoad™ PCR 100 bp Low Ladder, ready-to-use marker for DNA electrophoresis

F4135

Sérum de veau fœtal, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

P4333

Pénicilline-streptomycine, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

T3924

Solution de trypsine-EDTA, 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

D6171

Milieu d'Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

NA1020

GenElute™ PCR Clean-Up Kit, sufficient for 70 purifications

P9620

Puromycine dihydrochloride, Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

SH0111

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Kinases

SH0511

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Tumor Suppressors

SH0531

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Tumor Suppressors

SH1011

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Ion Channels

SH0811

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Cytokines and Chemokines

SH0411

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Phosphatases

SH1111

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Nuclear Hormone Receptors

SH1911

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Apoptosis Pathway

SH1311

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Cytokine and Chemokine Receptors

SH0211

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, G-Protein Coupled Receptors

SH0131

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Kinases

SH1031

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Ion Channels

SH0831

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Cytokines and Chemokines

SH0231

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, G-Protein Coupled Receptors

SH1131

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Nuclear Hormone Receptors

SH1331

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Cytokine and Chemokine Receptors

SH1931

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Apoptosis Pathway

SH0711

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Ubiquitin Hydrolases (DUBS)

SH2111

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Ubiquitin Ligases (E1,E2,E3)

SH1811

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, DNA Repair Pathway

SH2211

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Cell Adhesion Genes

SH2311

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Cytoskeleton Genes

SH2411

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, p53 Pathway

SH2511

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Extracellular Matrix Genes

SH2611

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Helicases

SH2711

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, JAK-STAT Pathway

SH2811

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, T-Cell Activation

SH2911

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, B-Cell Activation

SH3011

MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock, Epigenetic Regulators

SH0121

MISSION® shRNA Human Gene Family Set, DNA, Kinases

SH0221

MISSION® shRNA Human Gene Family Set, DNA, G-Protein Coupled Receptors

SH0421

MISSION® shRNA Human Gene Family Set, DNA, Phosphatases

SH0521

MISSION® shRNA Human Gene Family Set, DNA, Tumor Suppressors

SH0821

MISSION® shRNA Human Gene Family Set, DNA, Cytokines and Chemokines

SH1021

MISSION® shRNA Human Gene Family Set, DNA, Ion Channels

SH1121

MISSION® shRNA Human Gene Family Set, DNA, Nuclear Hormone Receptors

SH1321

MISSION® shRNA Human Gene Family Set, DNA, Cytokine and Chemokine Receptors

SH1921

MISSION® shRNA Human Gene Family Set, DNA, Apoptosis Pathway

SH0721

MISSION® shRNA Human Gene Family Set, DNA, Ubiquitin Hydrolases (DUBS)

SH2121

MISSION® shRNA Human Gene Family Set, DNA, Ubiquitin Ligases (E1,E2,E3)

SH1821

MISSION® shRNA Human Gene Family Set, DNA, DNA Repair Pathway

SH2221

MISSION® shRNA Human Gene Family Set, DNA, Cell Adhesion Genes

SH2321

MISSION® shRNA Human Gene Family Set, DNA, Cytoskeleton Genes

SH2421

MISSION® shRNA Human Gene Family Set, DNA, p53 Pathway

SH2521

MISSION® shRNA Human Gene Family Set, DNA, Extracellular Matrix Genes

SH2621

MISSION® shRNA Human Gene Family Set, DNA, Helicases

SH2721

MISSION® shRNA Human Gene Family Set, DNA, JAK-STAT Pathway

SH2821

MISSION® shRNA Human Gene Family Set, DNA, T-Cell Activation

SH2921

MISSION® shRNA Human Gene Family Set, DNA, B-Cell Activation

SH3021

MISSION® shRNA Human Gene Family Set, DNA, Epigenetic Regulators

SH0731

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Ubiquitin Hydrolases (DUBS)

SH2131

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Ubiquitin Ligases (E1,E2,E3)

SH1831

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, DNA Repair Pathway

SH2231

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Cell Adhesion Genes

SH2331

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Cytoskeleton Genes

SH2431

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, p53 Pathway

SH2531

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Extracellular Matrix Genes

SH2631

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Helicases

SH2731

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, JAK-STAT Pathway

SH2831

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, T-Cell Activation

SH2931

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, B-Cell Activation

SH3031

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Epigenetic Regulators

SH0431

MISSION® shRNA Human Gene Family Set, Lentiviral Particles, Phosphatases

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Rong Geng et al.
Chinese journal of cancer, 36(1), 63-63 (2017-08-02)
The mitogen-activated extracellular signal-regulated kinase 1/2 (MEK1/2) inhibitor trametinib has shown promising therapeutic effects on melanoma, but its efficacy on colorectal cancer (CRC) is limited. Synthetic lethality arises with a combination of two or more separate gene mutations that causes
Xue-Cen Wang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(14), 4567-4579 (2019-04-14)
Neoadjuvant chemoradiotherapy (neoCRT) is a standard treatment for locally advanced rectal cancer (LARC); however, resistance to chemoradiotherapy is one of the main obstacles to improving treatment outcomes. The goal of this study was to identify factors involved in the radioresistance
Ziyang Wang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(17), 2000593-2000593 (2020-10-01)
Colorectal cancer (CRC) has become a predominant cancer worldwide. To understand the process of carcinogenesis, a short hairpin RNA library screening is employed to search for candidate genes that promote proliferation in the CRC cell line HT29. The candidate genes
Davide Zecchin et al.
EMBO molecular medicine, 12(8), e11987-e11987 (2020-07-17)
Triple-negative breast cancer (TNBC) has poorer prognosis compared to other types of breast cancers due to the lack of effective therapies and markers for patient stratification. Loss of PTEN tumor suppressor gene expression is a frequent event in TNBC, resulting
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Afficher tous les articles scientifiques apparentés

Articles

Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Afficher tout

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