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Key Documents

SAB4500369

Sigma-Aldrich

Anti-Collagen IV antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

Arresten, COL4A1, Collagen α-1(IV) chain

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 160 kDa

Espèces réactives

human, mouse

Concentration

~1 mg/mL

Technique(s)

ELISA: 1:20000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... COL4A1(1282)

Catégories apparentées

Description générale

Anti-Collagen IV Antibody detects endogenous levels of total Collagen IV protein.
COL4A1 (collagen type IV alpha 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats. It is an important constituent of type IV collagen in the basement membrane. The COL4A1 gene is located on human chromosome 13q34.

Immunogène

The antiserum was produced against synthesized peptide derived from human Collagen IV.

Immunogen Range: 11-60

Application

Anti-Collagen IV, N-Terminal antibody has been used in histology analysis, immunofluorescence microscopy and immunostaining.

Actions biochimiques/physiologiques

COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Xiufeng Wu et al.
Oncology letters, 11(6), 3785-3789 (2016-06-18)
Since basement membranes represent a critical barrier during breast cancer progression, timely imaging of these signposts is essential for early diagnosis of breast cancer. A label-free method using multiphoton microscopy (MPM) based on two-photon excited fluorescence signals and second harmonic
Expression of mutant myocilin induces abnormal intracellular accumulation of selected extracellular matrix proteins in the trabecular meshwork.
Kasetti RB, et al.
Investigative Ophthalmology & Visual Science, 57(14), 6058-6069 (2016)
Laszlo Markasz et al.
BMC pulmonary medicine, 24(1), 75-75 (2024-02-09)
Collagen type IV alpha 1 chain (COL4A1) in the basement membrane is an important component during lung development, as suggested from animal models where COL4A1 has been shown to regulate alveolarization and angiogenesis. Less is known about its role in
Dongbo Zhao et al.
Endocrine journal, 68(7), 751-762 (2021-04-02)
Rosiglitazone (RSG) is widely used to reduce the amount of sugar in the blood of patients with diabetes mellitus. Diabetic nephropathy is the most common microvascular complication of diabetes. The role of RSG in diabetic nephropathy is not fully understood.
Effects of conventional immunosuppressive therapy on functional and pathological features of CNS lupus in NZB/W mice.
Alexander J, et al.
F1000Research, 4 (2018)

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