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SAB4200671

Sigma-Aldrich

Anti-S-100 (β-Subunit) antibody, Mouse monoclonal

clone SH-B1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, NEF, S100, S100-B, S100beta

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

SH-B1, monoclonal

Espèces réactives

cat, rabbit, porcine, bovine, rat, human

Concentration

~1 mg/mL

Technique(s)

immunohistochemistry: 1.5-3  μg/mL using immunoperoxidase labeling of pronase digested, formalin-fixed, paraffin-embedded sections of rabbit tongue.

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... S100B(6285)

Description générale

Monoclonal Anti-S-100 (β-subunit) (mouse IgG1 isotype) is derived from the SH-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. S-100 is a set of small, thermolabile, highly acidic homo or hetero-dimer calcium binding proteins. The protein exists in two isoforms namely, S-100α and S-100β, which are brain specific.
S-100β is a calcium binding protein. It is mainly present in astrocytes and neurons of hindbrain and spinal cord.

Immunogène

Purified bovine brain S-100β

Application

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in:
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA) (Ca2+ ion independent)
  • immunocytochemistry
  • immunoblotting
  • dot blot
  • immunohistochemistry.

Actions biochimiques/physiologiques

S-100 is involved in cell-growth regulation, increasing membrane permeability to cations, inflammatory response in many brain diseases, including schizophrenia, stimulation of nucleolar RNA polymerase activity and transporting proteins and free fatty acids in adipocytes. S-100β tissue distribution can be a useful tool in the differential diagnosis of neoplasms and proliferative processes.
S-100β protein interacts with synaptic, cytoskeletal and cell cycle proteins. Additionally, it can regulate calcium levels in glial and neuronal cells. It is involved in neuronal plasticity, astrogliosis and neuronal cell survival. S-100β is associated with Alzheimer disease and amyotrophic lateral sclerosis.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A Migheli et al.
Neuroscience letters, 261(1-2), 25-28 (1999-03-19)
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive motor neuron loss and astrogliosis. We studied the immunohistochemical expression of S-100beta, a calcium-binding protein with both neurotrophic and neurotoxic activities, in the spinal cord of patients with ALS.
Postnatal activation of TLR4 in astrocytes promotes excitatory synaptogenesis in hippocampal neurons
Shen Y, et al.
The Journal of cell biology, 215(5), 719-734 (2016)
Marc Oria et al.
Frontiers in molecular neuroscience, 15, 888351-888351 (2022-07-06)
During embryonic spinal cord development, neural progenitor cells (NPCs) generate three major cell lines: neurons, oligodendrocytes, and astrocytes at precise times and locations within the spinal cord. Recent studies demonstrate early astrogenesis in animal models of spina bifida, which may
Yan Guo et al.
Signal transduction and targeted therapy, 9(1), 32-32 (2024-02-14)
The appropriate and specific response of nerve cells to various external cues is essential for the establishment and maintenance of neural circuits, and this process requires the proper recruitment of adaptor molecules to selectively activate downstream pathways. Here, we identified
Yi Shen et al.
The Journal of cell biology, 215(5), 719-734 (2016-12-07)
Astrocytes are critical in synapse development, and their dysfunction in crucial developmental stages leads to serious neurodevelopmental diseases, including seizures and epilepsy. Immune challenges not only affect brain development, but also promote seizure generation and epileptogenesis, implying immune activation is
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