SAB4200095

Anti-PKM2 (isoform M2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-CTHBP, Anti-OIP3(OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~60 kDa
• Espèces réactives: human
• Concentration: ~1.5 mg/mL
• Technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon carcinoma; immunoprecipitation (IP): 2-4 μg using HT29 cell lysates; indirect immunofluorescence: 4-8 μg/mL using HeLa cells; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PKM2(5315)

Description

Description générale

Pyruvate Kinase (PK) has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis .

Spécificité

Anti-PKM2 (isoform M2) antibody is specific for human PKM2 (isoform M2). The product is expected to react with rat and mouse PKM2. In immunoblotting, detection of the PKM2 (isoform M2) band is specifically inhibited by the PKM2 (isoform M2) immunizing peptide.

Application

Anti-PKM2 (isoform M2) antibody produced in rabbit has been used in:

• chromatin immunoprecipitation (chip) assay
• western blotting
• immunofluorescence
• immunohistochemistry

Actions biochimiques/physiologiques

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable