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S8398

Sigma-Aldrich

Shields and Sang M3 Insect Medium

With L-glutamine, without potassium bicarbonate, powder, suitable for insect cell culture

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About This Item

Numéro MDL:
MFCD00243218
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Forme

powder

Technique(s)

cell culture | insect: suitable

Composants

NaHCO3: no
L-glutamine: 0.6 g/L
glucose: 10 g/L (Dextro)

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Shields and Sang M3 insect medium can support the rapid growth of both primary and established cultures of Drosophila melanogaster cells. Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects.

Application

Shields and Sang M3 Insect Medium has been used:
  • for the dissection of Drosophila larvae to study the changes in the translational activity of larval wing imaginal disc cells
  • to incubate Drosophila bodies for surface sensing of translation (SUnSET) assay
  • to culture Drosophila cells for transfection studies

Developed for the culture of Drosophila cell lines.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Louis Philip Benoit Bouvrette et al.
RNA (New York, N.Y.), 24(1), 98-113 (2017-10-29)
Cells are highly asymmetrical, a feature that relies on the sorting of molecular constituents, including proteins, lipids, and nucleic acids, to distinct subcellular locales. The localization of RNA molecules is an important layer of gene regulation required to modulate localized
Tim Hundertmark et al.
PloS one, 13(9), e0203622-e0203622 (2018-09-08)
Spermatogenesis in many species including Drosophila melanogaster is accompanied by major reorganisation of chromatin in post-meiotic stages, involving a nearly genome-wide displacement of histones by protamines, Mst77F and Protamine-like 99C. A proposed prerequisite for the histone-to-protamine transition is massive histone
Stefanie M K Gärtner et al.
European journal of cell biology, 94(1), 46-59 (2014-12-04)
Spermatogenesis is accompanied by a remarkable reorganization of the chromatin in post-meiotic stages, characterized by a near genome-wide displacement of histones by protamines and a transient expression of transition proteins. In Drosophila, the transition-protein-like protein Tpl94D contains an HMG-box domain
Ahmed Sabri et al.
PloS one, 6(4), e18847-e18847 (2011-04-29)
Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass rearing for inundative release is needed to improve its use in
Silke Rothenbusch-Fender et al.
Biology open, 6(12), 1876-1888 (2017-11-11)
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