R4654

Anti-Rab5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Ras-Related Protein Rab5A
• Numéro MDL: MFCD01322589
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~24 kDa
• Espèces réactives: human, mouse, rat
• Concentration: ~1 mg/mL
• Technique(s): indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells.; western blot: 2.5-5 μg/mL using whole extract of human A431 cells
• Numéro d'accès UniProt: P20339
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RAB5A(5868) ; mouse ... Rab5a(271457) ; rat ... Rab5a(64633)

Description

Description générale

Ras related protein consists of three isoforms such as Rab5a, Rab5b and Rab5c. Rab5a is encoded by the gene mapped to human chromosome 3p24.3. This gene codes for 23.66 kDa Rab5a protein.

Immunogène

synthetic peptide corresponding to amino acid residues 176-188 of human Rab5 with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Rab5 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Actions biochimiques/physiologiques

Rab proteins are members of small GTPases control the exocytosis and endocytosis and regulate vesicular transport. Rab5 regulates endocytic pathway in mammalian cells and an activator of autophagy. It mediates macroautophagy of cells expressing aggregates of mutant Huntingtin protein.

Rab5a is essential for epidermal growth factor (EGF)-mediated EGF receptor degradation. Overexpression of Rab5a is associated with the development of variety of cancers such as breast cancer, lung cancer, hepatocellular carcinoma (HCC) and ovarian cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable