P7937
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit
5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder
Synonyme(s) :
Protein phosphatase
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About This Item
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Produit recombinant
expressed in E. coli
Pureté
≥80% (GE)
Forme
lyophilized powder
Activité spécifique
5,000-15,000 units/mg protein
Poids mol.
37.5 kDa
Technique(s)
inhibition assay: suitable
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
rabbit ... PPP1CA(100009298)
Description générale
Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).
Application
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.
Actions biochimiques/physiologiques
Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).
Conditionnement
Package size based on protein content.
Définition de l'unité
One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.
Forme physique
Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer.
Inhibiteur
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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Les clients ont également consulté
Shota Takabatake et al.
Biochemical and biophysical research communications (2020-02-23)
Ca2+/calmodulin-dependent protein kinase kinase β (CaMKKβ) acts as a regulatory kinase that phosphorylates and activates multiple downstream kinases including CaMKI, CaMKIV, 5'AMP-activated protein kinase (AMPK) and protein kinase B (PKB), resulting in regulation of wide variety of Ca2+-dependent physiological responses
Serine/threonine phosphatases: mechanism through structure.
Shi Y
Cell, 139(3), 468-484 (2009)
A J Zhang et al.
The Journal of biological chemistry, 267(3), 1484-1490 (1992-01-25)
The catalytic subunit of rabbit skeletal muscle protein phosphatase-1 was expressed in Escherichia coli. Expression of phosphatase-1 in the pET3a vector, which is based on the use of the T7 promoter, resulted in the expression of the enzyme as an
Insulin resistance of glycogen synthase mediated by o-linked N-acetylglucosamine.
Parker GJ
The Journal of Biological Chemistry, 278(12), 10022-10027 (2003)
Satomi Ohtsuka et al.
Biochemistry, 59(17), 1701-1710 (2020-04-17)
Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) activates particular multifunctional kinases, including CaMKI, CaMKIV, and 5'AMP-activated protein kinase (AMPK), resulting in the regulation of various Ca2+-dependent cellular processes, including neuronal, metabolic, and pathophysiological pathways. We developed and characterized a novel pan-CaMKK inhibitor
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