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H5505

Sigma-Aldrich

Histone from calf thymus

Type III-S

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About This Item

Numéro CAS:
9064-47-5
Numéro Beilstein :
1895465
Numéro MDL:
MFCD00131329
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

Source biologique

bovine (calf) thymus

Niveau de qualité

200

Type

Type III-S

Forme

powder

Concentration

≥60% protein (biuret)

Technique(s)

activity assay: suitable

Solubilité

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

Température de stockage

2-8°C

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Application

Histone is suitable for use:
  • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
  • in Cdk5 activity assay
  • in histone H1 kinase assay
  • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
  • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Actions biochimiques/physiologiques

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Caractéristiques et avantages

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Autres remarques

Characterized as mainly subgroup f1 by SDS-PAGE

Notes préparatoires

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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