G2781
Anti-Glutamine Synthetase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonyme(s) :
Glutamine Synthetase Antibody, Glutamine Synthetase Antibody - Anti-Glutamine Synthetase antibody produced in rabbit
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
IgG fraction of antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen 45 kDa
Espèces réactives
rat
Conditionnement
antibody small pack of 25 μL
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections
microarray: suitable
western blot: 1:10,000 using rat brain cytosolic fraction
Numéro d'accès UniProt
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GLUL(2752)
mouse ... Glul(14645)
rat ... Glul(24957)
Description générale
Anti-Glutamine Synthetase is produced in rabbit using a synthetic peptide corresponding to the C-terminus of mouse glutamine synthetase (GS) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. This sequence is identical in human, bovine, rat, hamster and pig GS, and highly conserved in chicken GS. GS is found in mammals as an octamer of identical 45 kDa subunits. It is localized in glial cells of various species. It is present in the brain primarily in astrocytes and in the retina in Muller glial cells that support neurons using glutamate as a neurotransmitter. GS is found in several brain regions including hippocampus, cerebral cortex, neostriatum and cerebellar granular layer, corresponding to sites with high glutamate receptor density.
Spécificité
Anti-Glutamine synthetase recognizes rat glutamine synthetase (GS) (45 kDa). Staining of GS in immunoblotting is specifically inhibited with the GS immunizing peptide (amino acids 357-373 with Nterminally added lysine).
Immunogène
synthetic peptide corresponding to the C-terminal region of mouse glutamine synthetase (GS) (amino acids 357-373. This sequence is identical in human, bovine, rat, hamster, and pig GS and highly conserved chicken
Gs (single amino acid substitution).
Gs (single amino acid substitution).
Application
Immunoblotting: a minimum working antibody dilution of 1:10,000 is determined using a rat brain cytosolic fraction extract.
Immunohistochemistry: a minimum working antibody dilution of 1:10,000 is determined using formalin-fixed, paraffin-embedded sections of rat brain.
Anti-Glutamine Synthetase antibody has been used for the detection and localization of GS by immunoblotting and immunohistochemistry.
Actions biochimiques/physiologiques
Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. In the brain, GS is considered as a key enzyme, participating in recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification. GS activity is a useful marker for astrocytes and an important differentiation feature in the retina. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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