C2206

Anti-β-Catenin antibody produced in rabbit

whole antiserum

• Synonyme(s) : Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• Numéro MDL: MFCD02685970
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: whole antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: antigen 88-94 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: several mammalian species
• Conditionnement: antibody small pack of 25 μL
• Technique(s): dot blot: suitable using the immunogen and related peptides conjugated to BSA; immunocytochemistry: 1:2,000 using cultured MDBK cells; immunohistochemistry: 1:2,000 using bovine kidney frozen sections; western blot: 1:4,000 using cultured MDBK cells
• Application(s): research pathology
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Description

Description générale

Catenin are distinct peripheral cytosolic proteins and are classified into α-, β- and γ-catenin (102 kDa, 94 kDa and 86 kDa respectively. It is found in varying abundance in many developing and adult tissues.

Spécificité

The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).

Immunogène

synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.

Application

Anti-β-Catenin antibody produced in rabbit has been used in

• co-immunoprecipitation
• immunoblotting
• immunofluorescence
• immunoperoxidase assay

Actions biochimiques/physiologiques

Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The protein, β-catenin, binds directly to the cytoplasmic tail of E-cadherin. Plakoglobin (probably identical to γ-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene Adenomatous polyposis coli (APC). The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and wingless-related integration site (Wnt-1). p120cas which exhibits structural similarity to β-catenin and plakoglobin may represent another catenin associated with cadherin.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable