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Key Documents

B8395

Sigma-Aldrich

Anti-Bovine IgG (whole molecule) antibody produced in rabbit

whole antiserum

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

whole antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Contient

15 mM sodium azide

Technique(s)

indirect ELISA: 1:150,000
quantitative precipitin assay: 4.0 mg/mL

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Spécificité

The antiserum is determined to be immunospecific for bovine IgG.

Application

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used as a standard to determine protein content by Bradford method using Bio-Rad protein assay dye reagent. It has also been used in several assays to develop a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk.
Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used for detecting IgG antibodies in multichannel monolithic quartz crystal microbalance (MQCM).

Actions biochimiques/physiologiques

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulins G (IgGi, IgGa) of bovine milk is capable of protecting the gut mucosa against microorganisms.

Forme physique

Rabbit Anti-Bovine IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative

Notes préparatoires

treated to remove lipoproteins

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg


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Consulter la Bibliothèque de documents

Development of a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk
Jack NL, et al.
Journal of Agricultural and Food Chemistry, 41(4), 682-686 (1993)
Effect of thermal protectants on the stability of bovine milk immunoglobulin G
Chao-Cheng C and Hung-Min C
Journal of Agricultural and Food Chemistry, 46(9), 3570-3576 (1998)
Real-time multianalyte biosensors based on interference-free multichannel monolithic quartz crystal microbalance
Jaruwongrungsee K, et al.
Biosensors And Bioelectronics, 67, 576-581 (2015)
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG

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