602-05A

Hair Follicle Dermal Papilla Cells: HFDPC, adult

• Synonyme(s) : HFDPC, HFDPC cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human scalp (hair follicle papilla)
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Morphologie: mesenchymal
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): Alzheimer′s disease
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.

HFDPC have been used to demonstrate that IL-1α affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF (Boivin, 2006). They were also used to investigate how gene expression and cell proliferation are affected by testosterone analogues (Reiter, 2008), potential hair growth enhancing agents (Sasajima, 2008) and microbial metabolites (Takigawa, 2012). Using HFDPC, along with Human Epidermal Keratinocytes and Human Bronchial Epithelial Cells, scientists also studied mechanisms of cellular senescence, and develop ed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT (Haga, 2007). Finally, HFDPC have been used to discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1 (Watabe, 2013).

Origine de la lignée cellulaire

Scalp

Application

dermatalogical interactions, hair production, induction and maintenance of hair growth, hair follicle organization, development and evaluation of hair growth products, effects and secretion of growth factors and cytokines, gene expression, cell proliferation, cell senescence, cell lifespan extension, in vitro screening of androgen blocking agents, reservoir of multi-potent stem cells, skin reconstruction, wound healing, signal transduction pathways, skin composites and substitutes, hair follicle neogenesis

Composants

Cell Basal Medium containing 20% FBS & 5% DMSO

Notes préparatoires

• 2nd passage, >500,000 cells in Cell Basal Medium containing 20% FBS & 5% DMSO
• Can be cultured at least 6 doublings

Procédure de repiquage

Please refer to the HFDPC Culture Protocol.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable