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50983

Sigma-Aldrich

Guanidine thiocyanate solution

BioUltra, for molecular biology, ~6 M in H2O

Synonyme(s) :

Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Formule linéaire :
NH2C(=NH)NH2 · HSCN
Numéro CAS:
593-84-0
Poids moléculaire :
118.16
Numéro Beilstein :
3563461
Numéro MDL:
MFCD00013027
Code UNSPSC :
12352107
ID de substance PubChem :
57651292
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioUltra

Concentration

~6 M in H2O

Impuretés

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

Indice de réfraction

n20/D 1.474-1.483

pH

4.5-7.0 (25 °C)

Traces d'anions

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

Traces de cations

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

Absorption

cut-off at 280 nm

Chaîne SMILES 

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

Clé InChI

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.

Application

Guanidine thiocyanate solution has been used to extract:
  • plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
  • total RNA from collected tissues (shoots, flowers, fruits, and seeds)
  • in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)

Attention

possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Risques supp

EUH032

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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