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08124

Sigma-Aldrich

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥10,000,000 units/mg protein

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About This Item

Numéro de classification (Commission des enzymes):
3.2.1.68 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131418
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

ammonium sulfate suspension

Niveau de qualité

100

Activité spécifique

≥10,000,000 units/mg protein

Température de stockage

2-8°C

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Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Actions biochimiques/physiologiques

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Définition de l'unité

One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

Remarque sur l'analyse

≥10 MU/mg protein

Pictogrammes

Skull and crossbonesEnvironment
GHS06,GHS09

Mention d'avertissement

Danger

Mentions de danger

H302 + H332,H311,H411

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Enzymatic conversions of starch
Tomasik P and Horton D
Advances in Carbohydrate Chemistry and Biochemistry, 68, 59-436 (2012)
Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Roberto Manfredi et al.
International journal of STD & AIDS, 19(2), 99-105 (2008-03-13)
One thousand and eighty-one evaluable HIV-infected patients were assessed for pancreatic abnormalities in a prospective case-control study including the whole follow-up period of each patient (minimum 12 months). The 435 patients (40.2%), who experienced at least one episode of confirmed
Steven S-S Wang et al.
Bioprocess and biosystems engineering, 33(8), 1007-1015 (2010-04-08)
The current research examines the impact of agitation on deactivation of isoamylase and β-amylase under supercritical carbon dioxide (SC-CO2). Our experimental results showed that the activity of either enzyme decreased with increasing pressure or speed of agitation. The degree of
Lucio Gullo et al.
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 9(3), 240-244 (2009-05-02)
Among the various studies of pancreatic function in the elderly published so far, none have dealt with subjects over 90 years of age. The aim of this study was to examine pancreatic function in healthy individuals over 90 years old.
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