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KK3009

Roche

KAPA Taq EXtra PCR Kit

Synonyme(s) :

PCR, Taq

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 500 reactions

Niveau de qualité

100

Caractéristiques

High Fidelity PCR
Long Range PCR
dNTPs included
hotstart: no

Conditionnement

pkg of 250 U

Fabricant/nom de marque

Roche

Technique(s)

PCR: suitable

Entrée

purified DNA

Température de stockage

−20°C

Catégories apparentées

Application

The KAPA Taq EXtra PCR system is ideally suited for:
  • PCR of short- and medium-length targets (<5 kb)
  • Long-range PCR (5–15 kb)
  • PCR with limiting amounts of template DNA.

Actions biochimiques/physiologiques

The KAPA Taq EXtra PCR system is a blend of KAPA Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support robust, long range, and sensitive PCR. Both enzymes possess 5′→3′ polymerase activity, but only KAPA Taq DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. Reaction products generated with KAPA Taq EXtra are polyA-tailed, and can be cloned into TA-cloning vectors.

Caractéristiques et avantages

  • KAPA Taq EXtra is a blend of KAPA Taq and a proofreading DNA polymerase.
  • Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
  • Fidelity is 2-4 times better than Taq
  • For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
  • Amplify mid- to long-range targets with high yield and sensitivity.
  • KAPA Taq EXtra Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl2 is supplied separately for optimization.
  • The fidelity of KAPA Taq EXtra is 2-4 fold higher than that of KAPA Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases, such as KAPA HiFi.

Qualité

Each batch of KAPA Taq EXtra DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq EXtra PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Notes préparatoires

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Autres remarques

For Research Use Only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • KAPA Taq EXtra DNA Polymerase 5 U/µL
  • KAPA Taq EXtra Buffer (5X)
  • KAPA dNTP Mix 10 mM each
  • MgCl2 25 mM

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Pieranna Chiarella et al.
Future science OA, 5(2), FSO361-FSO361 (2019-03-02)
Gene polymorphism biomarkers identify individual susceptibility to environmental and occupational hazards. The conventional approach considers polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP), a reliable but expensive and time-consuming two-step procedure. Therefore we evaluated the simpler
Gloria Pérez-Rubio et al.
Diagnostics (Basel, Switzerland), 10(1) (2020-01-08)
Cigarette smoking is influenced by nicotine's effects on dopaminergic activity, which appear to be moderated by genetic variation, particularly a variable number tandem repeat (VNTR, 48 bp) polymorphism in the third exon of the dopamine receptor gene (DRD4). Smokers with

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