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11427598910

Roche

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

Synonyme(s) :

Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate

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About This Item

Formule empirique (notation de Hill):
C32H52N7O17P3S
Numéro CAS:
188755-42-2
Poids moléculaire :
931.78
Code UNSPSC :
41116100

Niveau de qualité

100

Pureté

≥98% (HPLC)

Forme

solution

Poids mol.

955.5 (biotin-16-ddUTP-Li4)

Conditionnement

pkg of 25 μL (25 nmol; 1mM)

Fabricant/nom de marque

Roche

Concentration

1 mM

Technique(s)

hybridization: suitable

Température de stockage

−20°C

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Description générale

Biotin-16-ddUTP, tetralithium salt; 1mM

Application

  • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
  • DNA polymerase I (Holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.
  • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Caractéristiques et avantages

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Qualité

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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