OP03
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
liquid, clone PAb421, Calbiochem®
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
PAb421, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
rabbit, monkey, human, mouse, rat
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Isotype
IgG2a
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TP53(7157)
Description générale
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Immunogène
Epitope: within amino acids 376-378 of human p53
Mouse
partially purified mouse p53
Application
Frozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Remarque sur l'analyse
Negative Control
SK-OV-3 cells or normal skin
SK-OV-3 cells or normal skin
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Autres remarques
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Rachel E Rempel et al.
PLoS genetics, 5(9), e1000640-e1000640 (2009-09-15)
The phenotypic heterogeneity that characterizes human cancers reflects the enormous genetic complexity of the oncogenic process. This complexity can also be seen in mouse models where it is frequently observed that in addition to the initiating genetic alteration, the resulting
Sricharan Bandhakavi et al.
Molecular & cellular proteomics : MCP, 9(2), 403-414 (2009-12-04)
Cellular nutritional and energy status regulates a wide range of nuclear processes important for cell growth, survival, and metabolic homeostasis. Mammalian target of rapamycin (mTOR) plays a key role in the cellular responses to nutrients. However, the nuclear processes governed
P J Grippo et al.
The American journal of pathology, 157(3), 805-813 (2000-09-12)
Transitional cell carcinoma (TCC), a neoplasm of urinary bladder urothelial cells, generally appears in either of two forms, papillary non-invasive or invasive TCC, although intermediate forms can occur. Each has a distinctive morphology and clinical course. Altered expression of the
Jessica Q Ho et al.
PloS one, 6(2), e16815-e16815 (2011-02-25)
The pro-survival activity of NF-κB in response to a variety of stimuli has been extensively characterized. Although there have been a few reports addressing the pro-cell death role of NF-κB, the precise mechanism of NF-κB's pro-cell death function still remains
Sun-Young Choi et al.
International journal of oncology, 40(1), 277-286 (2011-09-03)
Proteins involved in the G1 phase of the cell cycle are aberrantly expressed, sometimes in mutated forms, in human cancers including human hepatocellular carcinoma.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique