MABF2105

Anti-CD6 Antibody, clone UMCD6

clone UMCD6, from mouse

• Synonyme(s) : T-cell differentiation antigen CD6, T12, TP120
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: UMCD6, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): dot blot: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_006716
• Numéro d'accès UniProt: P30203
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CD6(923)

Description

Description générale

T-cell differentiation antigen CD6 (UniProt: P30203; also known as T12, TP120, CD6) is encoded by the CD6 gene (Gene ID: 923) in human. CD6 is a single-pass type I membrane protein that serves as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It contributes to signaling cascades triggered by activation of the TCR/CD3 complex and functions as a costimulatory molecule to promote T-cell activation and proliferation. It is also involved in the formation and maturation of the immunological synapse. CD6 has been detected on peripheral blood T-cells and on natural killer (NK) cells and is also detected in spleen, thymus, appendix, lymph nodes. CD6 is known to bind both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria and LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 18-402), a transmembrane domain (aa 403-423), and a cytoplasmic domain (aa 424-668). Seven isoforms of CD6 have been reported that are produced by alternative splicing.

Spécificité

Clone UMCD6 specifically detects CD6 in human cells.

Immunogène

ST-1 T cells isolated from human knee synovium.

Application

Anti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in Dot Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay.

Inhibition Analysis: A representative lot inhibited antigen-specific responses to tetanus toxoid, but not to the superantigen SEA. (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43).

Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Dot Blot Analysis: A representative lot detected CD6 in Dot Blot applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. al. (1994). J Immunol. 153(1):1-9; Li, Y., et. al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692).

Qualité

Evaluated by Flow Cytometry in PMA stimulated Jurkat cells.

Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA.

Description de la cible

71.80 kDa calculated.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.