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Key Documents

MAB5320

Sigma-Aldrich

Anti-Synuclein α Antibody, clone 7B12.2

culture supernatant, clone 7B12.2, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

culture supernatant

Type de produit anticorps

primary antibodies

Clone

7B12.2, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SNCA(6622)

Spécificité

Reacts with alpha Synuclein. By Western blot the antibody shows no reactivity with beta or gamma synuclein. This antibody was shown to stain Lewy bodies in frozen section of human brain with Parkinson′s disease and glial inclusions in frozen section of human brain with Multiple System Atrophy.

Immunogène

Recombinant human alpha synuclein.

Application

Anti-Synuclein Antibody, α, clone 7B12.2 detects level of Synuclein & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blotting on recombinant alpha synuclein: 1:2,000-1:4,000.

Lane 1: Recombinant human alpha synuclein

Lane 2: Recombinant human beta synuclein

Lane 3: Recombinant human gamma synuclein

Immunohistochemistry (frozen sections): 1:250-1:500

Optimal working dilutions must be determined by end user.

Description de la cible

15-18 kDa

Liaison

Replaces: 04-1053

Forme physique

UnPurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Unpurified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Brain tissue

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Nanoscale imaging of individual amyloid aggregates extracted from brains of Alzheimer and Parkinson patients reveals presence of lipids in I?-synuclein but not in amyloid I?1-42 fibrils.
Zhaliazka, et al.
Protein Science, 32, e4598-e4598 (2023)
Francesca Sardone et al.
Journal of cellular physiology, 229(7), 878-886 (2013-12-21)
Cell-extracellular matrix interaction plays a major role in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. Collagen VI is a widely expressed non-fibrillar collagen, which regulates tissues homeostasis. The objective of the
Xichen Zheng et al.
The Journal of clinical investigation, 128(5), 2104-2115 (2018-04-18)
Immune checkpoint blockade (ICB) has demonstrated curative potential in several types of cancer, but only for a small number of patients. Thus, the identification of reliable and noninvasive biomarkers for predicting ICB responsiveness is an urgent unmet need. Here, we
Francesca Sardone et al.
Matrix biology : journal of the International Society for Matrix Biology, 55, 90-105 (2016-10-19)
In response to injury, tendon fibroblasts are activated, migrate to the wound, and contribute to tissue repair by producing and organizing the extracellular matrix. Collagen VI is a microfibrillar collagen enriched in the pericellular matrix of tendon fibroblasts with a
Lu-Yao Tong et al.
Frontiers in pharmacology, 13, 914153-914153 (2022-07-23)
The recovery of spinal cord injury (SCI) is closely associated with the obstruction of oligodendrocyte progenitor cell (OPC) differentiation, which ultimately induces the inability to generate newly formed myelin. To address the concern, drug-based methods may be the most practical

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