MAB343
Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4
ascites fluid, clone 2.F2.19B4, Chemicon®
Synonyme(s) :
APP, Jonas clone
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
ascites fluid
Clone
2.F2.19B4, monoclonal
Espèces réactives
rat, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... APP(351)
Spécificité
Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html
Immunogène
Carboxyl fragment of APP 643-695 / Jonas.
Epitope: APP 643-695 C-terminal fragment
Application
Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.
Western blot: 10 μg/mL
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB343
IMMUNOHISTOCHEMISTRY
1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).
2) Wash sections 3 times in distilled water.
3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.
4) Wash sections for 5 min in TBS.
5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.
6) Wash sections 3 times in TBS for 5 min..
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in TBS.
9) Embed sections and examine.
Western blot: 10 μg/mL
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB343
IMMUNOHISTOCHEMISTRY
1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).
2) Wash sections 3 times in distilled water.
3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.
4) Wash sections for 5 min in TBS.
5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.
6) Wash sections 3 times in TBS for 5 min..
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in TBS.
9) Embed sections and examine.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.
Forme physique
Liquid.
Unpurified
Stockage et stabilité
Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
Brain
Brain
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
En option
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis.
Quan-Hong Ma, Toshitaka Futagawa, Wu-Lin Yang, Xiao-Dan Jiang, Li Zeng, Yasuo Takeda et al.
Nature Cell Biology null
David Marks et al.
Acta neuropathologica communications, 9(1), 66-66 (2021-04-15)
The amyloid precursor protein (APP) is a type I transmembrane protein with unknown physiological function but potential impact in neurodegeneration. The current study demonstrates that APP signals to the nucleus causing the generation of aggregates consisting of its adapter protein
Eun Mi Hwang et al.
Bioorganic & medicinal chemistry, 16(14), 6669-6674 (2008-06-21)
In order to access beta-secretase (BACE1), and enzyme strongly implicated in the cause of Alzheimer's disease, inhibitors must possess sufficient lipophilicity to traverse two lipid bilayers. Current drug candidates, which are almost totally peptide-derived, are thus inefficient because cell permeability
Processing of the amyloid protein precursor to potentially amyloidogenic derivatives.
Golde, T E, et al.
Science (New York, N.Y.), 255, 728-730 (1992)
Amyloid beta-peptide is produced by cultured cells during normal metabolism.
Haass, C, et al.
Nature, 359, 322-325 (1992)
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