MAB16985F
Anti-MCAM Antibody, clone P1H12, FITC conjugated
clone P1H12, Chemicon®, from mouse
Synonyme(s) :
MUC18, CD146
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
FITC conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
P1H12, monoclonal
Espèces réactives
canine, mouse, human
Ne doit pas réagir avec
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MCAM(4162)
Description générale
MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
Spécificité
Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation.
Immunogène
Immunization of mice with HUVEC
Application
Detect MCAM using this Anti-MCAM Antibody, clone P1H12, FITC conjugated validated for use in FC.
Immunocytochemistry: 1-10 μg/mL. Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry: 1-10 μg/mL. 4% PFA for 30min RT or <2hrs @4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed.
Facs Analysis: 1-10 μg/mL
Optimal working dilutions and protocols must be determined by end user.
Immunohistochemistry: 1-10 μg/mL. 4% PFA for 30min RT or <2hrs @4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed.
Facs Analysis: 1-10 μg/mL
Optimal working dilutions and protocols must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Forme physique
Protein A purified
Purified immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH=7.6, 15 mg/nL BSA containing 0.1% sodium azide as a preservative.
Stockage et stabilité
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
HUVEC cells
HUVEC cells
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Ramalingam, SS; Belani, CP; Mack, PC; Vokes, EE; Longmate, J; Govindan, R; Koczywas et al.
Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer null
Stefania Elena Navone et al.
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Stefania E Navone et al.
Nature protocols, 8(9), 1680-1693 (2013-08-10)
Brain microvascular endothelial cells (BMVECs) have an important role in the constitution of the blood-brain barrier (BBB). The BBB is involved in the disease processes of a number of neurological disorders in which its permeability increases. Isolation of BMVECs could
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