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Merck
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Key Documents

EZRL

Millipore

Rat Leptin ELISA Kit

measures and quantifies Leptin levels in 10 μL plasma or serum

Synonyme(s) :

Leptin, Obesity Factor

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.32

product name

Rat Leptin ELISA, This Rat Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine research.

Niveau de qualité

200

Espèces réactives

rat

Conditionnement

kit of 1 × 96 wells

Paramètres

10 μL sample volume (4hr assay)

assay range

accuracy: 102-122%
linearity: 87-100%
sensitivity: 0.04 ng/mL

Technique(s)

ELISA: suitable

Entrée

sample type plasma (K2 EDTA)
sample type serum

Numéro d'accès UniProt

P41160
P50596

Application(s)

research use

Méthode de détection

colorimetric (450nm/590nm)

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Leptin is an adipokine secreted by the adipose tissue. It is also found in the primary and secondary lymphoid organs. Leptin has a 3D structure made up of four α-helix bundle motifs. It participates in regulating neuroendocrine function and energy homeostasis. Leptin also participates in physiological processes such as reproduction, angiogenesis, and hemopoiesis. Rat Leptin enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of leptin in rat sera. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user.

Application

Rat Leptin ELISA kit has been used in the determination of leptin from rat serum and plasma samples.

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Warning

Mentions de danger

H290,H317

Classification des risques

Met. Corr. 1 - Skin Sens. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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Consulter la Bibliothèque de documents

Staci D Bilbo et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(6), 2104-2115 (2010-02-04)
Obesity is well characterized as a systemic inflammatory condition, and is also associated with cognitive disruption, suggesting a link between the two. We assessed whether peripheral inflammation in maternal obesity may be transferred to the offspring brain, in particular, the
Effects of high-fat high-sucrose feeding, energy restriction, and trans-10,cis-12 conjugated linoleic acid on visfatin and apelin in hamsters.
Arrate Lasa,Itziar Churruca,Edurne Simon,Maria Teresa Macarulla et al.
Journal of the American College of Nutrition null
Giuseppe Matarese et al.
Journal of immunology (Baltimore, Md. : 1950), 174(6), 3137-3142 (2005-03-08)
Leptin is an adipokine which conveys information on energy availability. In humans, leptin influences energy homeostasis and regulates neuroendocrine function primarily in states of energy deficiency. As a cytokine, leptin also affects thymic homeostasis and, similar to other proinflammatory cytokines
Anxiety-like behaviour and associated neurochemical and endocrinological alterations in male pups exposed to prenatal stress.
Charlotte Laloux,Jerome Mairesse,Gilles Van Camp,Angela Giovine,Igor Branchi et al.
Psychoneuroendocrinology null
Bérengère Coupé et al.
PloS one, 7(1), e30616-e30616 (2012-02-01)
Intrauterine growth restriction (IUGR) is closely linked with metabolic diseases, appetite disorders and obesity at adulthood. Leptin, a major adipokine secreted by adipose tissue, circulates in direct proportion to body fat stores, enters the brain and regulates food intake and
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