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Merck
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Key Documents

16-218

Sigma-Aldrich

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 conjugate

clone 3H11, Upstate®, from mouse

Synonyme(s) :

H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Conjugué

CY5 conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

3H11, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Upstate®

Technique(s)

immunofluorescence: suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NM_003493.2

Numéro d'accès UniProt

Q16695

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pSer10)

Informations sur le gène

human ... HIST1H3F(8968)

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spécificité

Expected to cross react with all vertebrate species.
Histone H3 phosphorylated at Ser10

Immunogène

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806.

Application

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 Conjugate is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Cy5.5 NHS ester (Cy5) and validated in IF, WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Qualité

Routinely evaluated by immunoblot in acid extrated protein from mitotic HeLa cells treated with colcemid (17-306).

Description de la cible

17kDa

Forme physique

Protein G Purified

Stockage et stabilité

1 year at 4°C from date of shipment

Remarque sur l'analyse

Control
Acid extracts from colcemid treated HeLa cells (lane 2)

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Jorge Boucas et al.
PloS one, 10(5), e0125745-e0125745 (2015-05-21)
Growing evidence suggests a key role for RNA binding proteins (RBPs) in genome stability programs. Additionally, recent developments in RNA sequencing technologies, as well as mass-spectrometry techniques, have greatly expanded our knowledge on protein-RNA interactions. We here use full transcriptome

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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