Skip to Content
Merck
  • Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth.

Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth.

Proceedings of the National Academy of Sciences of the United States of America (2013-12-25)
Yin Wang, Ko Noguchi, Natsuko Ono, Shin-ichiro Inoue, Ichiro Terashima, Toshinori Kinoshita
ABSTRACT

Stomatal pores surrounded by a pair of guard cells in the plant epidermis control gas exchange between plants and the atmosphere in response to light, CO2, and the plant hormone abscisic acid. Light-induced stomatal opening is mediated by at least three key components: the blue light receptor phototropin (phot1 and phot2), plasma membrane H(+)-ATPase, and plasma membrane inward-rectifying K(+) channels. Very few attempts have been made to enhance stomatal opening with the goal of increasing photosynthesis and plant growth, even though stomatal resistance is thought to be the major limiting factor for CO2 uptake by plants. Here, we show that transgenic Arabidopsis plants overexpressing H(+)-ATPase using the strong guard cell promoter GC1 showed enhanced light-induced stomatal opening, photosynthesis, and plant growth. The transgenic plants produced larger and increased numbers of rosette leaves, with ∼42-63% greater fresh and dry weights than the wild type in the first 25 d of growth. The dry weights of total flowering stems of 45-d-old transgenic plants, including seeds, siliques, and flowers, were ∼36-41% greater than those of the wild type. In addition, stomata in the transgenic plants closed normally in response to darkness and abscisic acid. In contrast, the overexpression of phototropin or inward-rectifying K(+) channels in guard cells had no effect on these phenotypes. These results demonstrate that stomatal aperture is a limiting factor in photosynthesis and plant growth, and that manipulation of stomatal opening by overexpressing H(+)-ATPase in guard cells is useful for the promotion of plant growth.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
Sigma-Aldrich
Activated Charcoal Norit®, Norit® CA1, wood, chemically activated, powder
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
Sigma-Aldrich
Carbon dioxide, ≥99.8%
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
Sigma-Aldrich
Carbon-12C dioxide, 99.99 atom % 12C
Sigma-Aldrich
Carbon, nanopowder, <100 nm particle size (TEM)
Millipore
Activated charcoal, suitable for GC
Supelco
Activated charcoal, powder
Supelco
Activated Charcoal Norit®, Norit® RBAA-3, rod
Sigma-Aldrich
Carbon-12C dioxide, 99.9 atom % 12C
Sigma-Aldrich
Activated charcoal, suitable for cell culture, suitable for plant cell culture
Supelco
Activated charcoal, for the determination of AOX, 50-150 μm particle size
Supelco
Activated charcoal, puriss. p.a., powder
Sigma-Aldrich
Carbon, glassy, spherical powder, 2-12 μm, 99.95% trace metals basis
Sigma-Aldrich
Activated charcoal, DARCO®, 12-20 mesh, granular
Sigma-Aldrich
Activated charcoal, DARCO®, 20-40 mesh particle size, granular
Sigma-Aldrich
Activated charcoal, DARCO®, −100 mesh particle size, powder
Sigma-Aldrich
Activated charcoal, Norit® ROW 0.8 SUPRA, pellets
Sigma-Aldrich
Activated charcoal, DARCO®, 4-12 mesh particle size, granular
Sigma-Aldrich
Activated charcoal, powder, -100 particle size (mesh), decolorizing
Sigma-Aldrich
Activated charcoal, untreated, granular, 20-60 mesh
Sigma-Aldrich
Activated charcoal, acid-washed with hydrochloric acid
Sigma-Aldrich
Activated charcoal, acid-washed with phosphoric and sulfuric acids
Sigma-Aldrich
Activated charcoal, untreated, granular, ≤5 mm
Sigma-Aldrich
Activated charcoal, untreated, granular, 8-20 mesh
Sigma-Aldrich
Activated Charcoal, meets USP testing specifications