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  • Targeted lipidomics and transcriptomics profiling reveal the heterogeneity of visceral and subcutaneous white adipose tissue.

Targeted lipidomics and transcriptomics profiling reveal the heterogeneity of visceral and subcutaneous white adipose tissue.

Life sciences (2020-02-02)
Biyu Hou, Yan Zhao, Ping He, Chunyang Xu, Peng Ma, Sin Man Lam, Bowen Li, Victoria Gil, Guanghou Shui, Guifen Qiang, Chong Wee Liew, Guanhua Du
ABSTRACT

The depot-specific differences in lipidome of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) reflect heterogeneity of white adipose tissue (WAT), which plays a central role in its distinct response to outside stimuli. However, the detailed lipidome of depot-specific WAT is largely unknown, especially the minor constitutes including phospholipid and sphingolipid. To investigate this field, we applied a high-coverage targeted lipidomics approach of VAT and SAT in male C57BL/6J mice to compare the basal level of their lipid profiles. Applying microarray and quantitative real-time polymerase chain reaction, we analyzed the transcriptome of twodepot-specific WAT and verified the differences in individual genes. In total, 342 lipid species from 19 lipid classes were identified. Our results showed the composition of TAG and FFA were different in length of chain and saturation. Interestingly, low abundance phospholipid, sphingolipid and cardiolipin were significantly higher in SAT. Lipid correlation network analysis vindicated that TAG and phospholipid formed distinct subnet and had more connections with other lipid species. Enriched ontology analysis of gene screened from LIPID MAPS and microarray suggested the differences were mainly involved in lipid metabolism, insulin resistance and inflammatory response. Our comprehensive lipidomics and transcriptomics analyses revealed differences in lipid composition and lipid metabolism of two depot-specific WAT, which would offer new insights into the investigation of heterogeneity of visceral and subcutaneous white adipose tissue.

MATERIALS
Product Number
Brand
Product Description

Avanti
17:1 Lyso PS, 1-(10Z-heptadecenoyl)-2-hydroxy-sn-glycero-3-[phospho-L-serine] (sodium salt), chloroform
Avanti
C8 Glucosyl(β) Ceramide (d18:1/8:0), Avanti Research - A Croda Brand 860540P, powder
Avanti
17:1 Lyso PS, 1-(10Z-heptadecenoyl)-2-hydroxy-sn-glycero-3-[phospho-L-serine] (sodium salt), powder
Avanti
C8 Galactosyl(β) Ceramide (d18:1/8:0), Avanti Research - A Croda Brand 860538P, powder
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17:1 Lyso PE, 1-(10Z-heptadecenoyl)-sn-glycero-3-phosphoethanolamine, chloroform
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C17 Ceramide (d18:1/17:0), Avanti Research - A Croda Brand