Skip to Content
Merck
All Photos(1)

Key Documents

G005515

HPLC Analysis of Thyroxine Enantiomers on Astec® CHIROBIOTIC® TAG

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase: 1% ammonium formate in methanol
flow rate: 1 mL/min
pressure: 826 psi (57 bar)
column temp.: 25 °C
detector: UV, 220 nm
injection: 10 μL
sample: each enantiomer 0.1 mg/L in methanol

suitability

application for HPLC

application(s)

clinical
life science and biopharma

Analysis Note

Baseline resolution of the chiral enantiomers of DL-Thyroxine is achieved by polar ionic mode on the Astec CHIROBIOTIC TAG with the use of an LC/MS-compatible mobile phase.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    L-Thyroxine, ≥98% (HPLC)
    D-Thyroxine, 99%

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service