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Key Documents

66830-U

Supelco

BIOshell A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 0.5 cm × 2.1 mm, HPLC Guard Cartridge, pkg of 3 ea

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A400 Protein C4, 3.4 μm Guard Cartridge, 3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

material

stainless steel hardware (guard column)

Quality Level

Agency

suitable for USP L26

description

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

product line

BIOshell

feature

endcapped

packaging

pkg of 3 ea

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

0.5 cm × 2.1 mm

surface area

15 m2/g

surface coverage

surface coverage 4.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

BIOshell Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of BIOshell columns. These BIOshell guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridge is packed with BIOshell Fused-Core particles. Order the guard column holder (66841-U) separately.

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Articles

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Protocols

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

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