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Key Documents

12-500

Sigma-Aldrich

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

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About This Item

UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.42

biological source

human

Quality Level

form

liquid

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

shipped in

dry ice

General description

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

Cell Line Description

HeLa

Physical form

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

Storage and Stability

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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April A Dukes et al.
Journal of neurochemistry, 106(1), 333-346 (2008-04-04)
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Christoph E Joosten et al.
Biotechnology progress, 19(3), 739-749 (2003-06-07)
Secreted human placental alkaline phosphatase (SEAP) was produced in a nonengineered Trichoplusia ni insect cell line, Tn-4s, using a recombinant Autographa californica baculovirus expression vector. The effect of culture conditions on SEAP specific yield and glycosylation was studied. When cultured
Gillian A Scullion et al.
Journal of Alzheimer's disease : JAD, 34(3), 781-793 (2013-01-11)
A lifestyle rich in physical and mental activities protects against Alzheimer's disease (AD) but the underlying mechanisms are unclear. We have proposed that this is mediated by a stress response and have shown that repeated exposure to novelty stress, which
Ioannis Partheniadis et al.
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