Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

357634

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

268259

Sigma-Aldrich

Nickel, foil, thickness 0.125 mm, ≥99.9%

267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

267058

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

268283

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

203904

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

510068

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 3202, slurry, in H₂O, active catalyst

510041

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 4200, slurry, in H₂O, active catalyst

510033

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2400, slurry, in H₂O, active catalyst

221678

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2800, slurry, in H₂O, active catalyst

GF18961960

Nickel, tube, 200mm, outside diameter 1.3mm, inside diameter 1.1mm, wall thickness 0.1mm, hard, 99.5%

GF05980754

Nickel, tube, 500mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%

GF28279840

Nickel, foil, 25mm disks, thickness 0.008mm, 99.95%

GF95347110

Nickel, tube, 500mm, outside diameter 0.762mm, inside diameter 0.204mm, wall thickness 0.279mm, hard, 99.5%

GF97622815

Nickel, tube, 200mm, outside diameter 2.5mm, inside diameter 2.25mm, wall thickness 0.125mm, hard, 99.5%

GF97079912

Nickel, wire reel, 5m, diameter 0.4mm, as drawn, 99.99+%

GF95500593

Nickel, wire reel, 50m, diameter 0.4mm, as drawn, 99%

GF80039538

Nickel, wire reel, 2m, diameter 0.5mm, hard, 99.98%

GF46862332

Nickel, foil, 25mm disks, thickness 0.0025mm, 99.95%

GF01529423

Nickel, wire reel, 5m, diameter 0.75mm, as drawn, 99%

GF39747224

Nickel, wire reel, 50m, diameter 2.0mm, hard, 99.98%

GF93039633

Nickel, wire reel, 200m, diameter 0.125mm, annealed, 99.98%

GF15429595

Nickel, tube, 200mm, outside diameter 6.5mm, inside diameter 5.5mm, wall thickness 0.5mm, hard, 99.5%

GF26431871

Nickel, wire reel, 20m, diameter 0.125mm, as drawn, 99%

GF65063628

Nickel, foil, 25mm disks, thickness 0.009mm, 99.95%

GF54898419

Nickel, tube, 200mm, outside diameter 2.5mm, inside diameter 2mm, wall thickness 0.25mm, hard, 99.5%

GF06866770

Nickel, tube, 500mm, outside diameter 0.78mm, inside diameter 0.2mm, wall thickness 0.29mm, as drawn, 99.5%

GF97647845

Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%

GF64122094

Nickel, wire reel, 250m, diameter 0.125mm, as drawn, 99%

GF11232694

Nickel, wire reel, 5m, diameter 0.25mm, as drawn, 99%