- A quantitative liposome microarray to systematically characterize protein-lipid interactions.
A quantitative liposome microarray to systematically characterize protein-lipid interactions.
Nature methods (2013-11-26)
Antoine-Emmanuel Saliba, Ivana Vonkova, Stefano Ceschia, Greg M Findlay, Kenji Maeda, Christian Tischer, Samy Deghou, Vera van Noort, Peer Bork, Tony Pawson, Jan Ellenberg, Anne-Claude Gavin
PMID24270602
ABSTRACT
Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.
MATERIALS
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Sigma-Aldrich
Sepharose™ 6B, 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)