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  • "Green odor" inhalation by stressed rat dams reduces behavioral and neuroendocrine signs of prenatal stress in the offspring.

"Green odor" inhalation by stressed rat dams reduces behavioral and neuroendocrine signs of prenatal stress in the offspring.

Hormones and behavior (2010-03-20)
Sayaka Fujita, Shiori Ueki, Michio Miyoshi, Tatsuo Watanabe
ABSTRACT

Chronic maternal stress during pregnancy results in the "prenatally stressed" offspring displaying behavioral and neuroendocrine alterations that persist into adulthood. We investigated how inhalation of green odor (a mixture of equal amounts of trans-2-hexenal and cis-3-hexenol) by stressed dams might alter certain indices of prenatal stress in their offspring. These indices were depression-like behavior (increased immobility time in the forced-swim test) and acute restraint stress-induced changes in hypothalamo-pituitary-adrenocortical (HPA) axis activity [plasma corticosterone (CORT) and ACTH levels and the number of Fos-immunoreactive cells in the hypothalamic paraventricular nucleus (an index of neuronal activity)]. Pregnant rats were exposed to restraint stress for 60 min/day for 10 days (gestational days 10-19). The prenatally stressed offspring exhibited significant increases in depression-like behavior and in restraint stress-induced ACTH, CORT, and Fos responses, unless their dam had been exposed to green odor. The behavioral effect of the odor was also seen in offspring that were fostered by unstressed dams. The results obtained in the dams themselves were as follows. In vehicle-exposed stressed dams, but not in green odor-exposed ones, total body and adrenal weights were significantly decreased or increased, respectively. Depression-like behavior was not observed in the vehicle-exposed stressed dams themselves. Green odor inhalation prevented the impairment of maternal behavior induced by restraint stress. Thus, exposure of dams to stress may affect both the fetal brain and fetal HPA axis, and also maternal behavior, leading to altered behavioral and neuroendocrine responses in the offspring. Such effects may be prevented by the stressed dams inhaling green odor.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-3-Hexen-1-ol, 97%
Sigma-Aldrich
trans-3-Hexen-1-ol, ≥95%, stabilized
Supelco
cis-3-Hexen-1-ol, analytical standard
Sigma-Aldrich
trans-2-Hexen-1-al, 98%
Sigma-Aldrich
cis-3-Hexen-1-ol, 98%
Sigma-Aldrich
trans-2-Hexen-1-al, ≥95%, FCC, FG
Sigma-Aldrich
cis-3-Hexen-1-ol, natural, >98%, FCC, FG