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Key Documents

P5306

Sigma-Aldrich

Papain from papaya latex

lyophilized powder, aseptically filled

Synonym(s):

Papainase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

papaya (latex)

Quality Level

sterility

aseptically filled

form

lyophilized powder

specific activity

≥8.0 units/mg protein

mol wt

23.4 kDa

solubility

H2O: soluble 1.2 mg/mL

storage temp.

−20°C

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General description

Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.

Application

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR).
The product from Sigma has been used in the digestion of extracellular matrix (ECM). It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.

Preparation Note

Prepared from P 4762

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Improving thermostability of papain through structure-based protein engineering
Choudhury D, et al.
Protein engineering, design & selection : PEDS, 23(6), 457-467 (2010)
Clara Malo et al.
Andrologia, 53(11), e14224-e14224 (2021-09-09)
This study evaluated the effects of freezing extenders, cryoprotectants and their concentrations, presence of Orvus Es Paste and freezing rates for cryopreserving dromedary camel sperm. Semen (five males; 2 ejaculates/male) was frozen in one of the following extenders (Green Buffer®
Amy Wernimont et al.
PloS one, 4(4), e5094-e5094 (2009-04-09)
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13-15 crystallize in a form that enables structure determination. In 2007, Dong et al reported that the addition of trace amounts
M van Vijven et al.
Journal of the mechanical behavior of biomedical materials, 126, 105010-105010 (2021-12-14)
A ruptured anterior cruciate ligament (ACL) is often reconstructed with a multiple-strand autograft of a semitendinosus tendon alone or combined with a gracilis tendon. Up to 10% of patients experience graft rupture. This potentially results from excessive local tissue strains
Gaetano R Barile et al.
Investigative ophthalmology & visual science, 46(8), 2916-2924 (2005-07-27)
The receptor for advanced glycation end products (AGEs) has been implicated in the pathogenesis of diabetic complications. This study was conducted to characterize the role of the RAGE axis in a murine model of nonproliferative diabetic retinopathy (NPDR). The retinas

Articles

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

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