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Key Documents

H0527

Sigma-Aldrich

HEPES potassium salt

≥99.5% (titration)

Synonym(s):

Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-monopotassium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17KN2O4S
CAS Number:
Molecular Weight:
276.39
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Assay

≥99.5% (titration)

form

powder

useful pH range

6.8-8.2

pKa 

7.5

solubility

water: 0.5 g/mL, clear, colorless

SMILES string

[K+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

KWURIFKZTJGMFD-UHFFFAOYSA-M

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General description

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a widely used Good’s buffer in biochemical and biological research applications. As a zwitterionic buffer, it stabilizes the pH of the solution even when small amounts of acid or base are added.

Application

HEPES potassium salt has been used as a buffering agent in nanopore fabrication and electrolyte preparations. It has also been used as a component of cell lysis buffer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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