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B5926

Sigma-Aldrich

10× REDTaq® PCR Reaction Buffer

To be used with REDTaq® DNA Polymerase

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About This Item

UNSPSC Code:
41106306
NACRES:
NA.52

Quality Level

form

liquid

color

colorless

storage temp.

−20°C

General description

10x REDTaq® PCR Reaction Buffer is a polymerase chain reaction buffer for use with REDTaq® DNA Polymerase (D4309).

Application

10× REDTaq® PCR Reaction Buffer has been used as a component of reaction mix:

  • for PCR detection of virulence genes from Campylobacter jejuni food and clinical isolates in BALB/c mice
  • for PCR detection of virulence genes of Campylobacter coli isolates from infected mice liver
  • for identifying K-ras mutated alleles by PCR-restriction fragment length polymorphism (RFLP) in patients with colorectal carcinoma

Legal Information

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Erin M Symonds et al.
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Human fecal matter contains a large number of viruses, and current bacterial indicators used for monitoring water quality do not correlate with the presence of pathogenic viruses. Adenoviruses and enteroviruses have often been used to identify fecal pollution in the
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Epigenetic information includes heritable signals that modulate gene expression but are not encoded in the primary nucleotide sequence. We have studied natural epigenetic variation in three allotetraploid sibling orchid species (Dactylorhiza majalis s.str, D. traunsteineri s.l., and D. ebudensis) that
Christoph P Dieterle et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 10(2), 641-650 (2004-02-05)
The aim of this study was to identify K-ras mutations as marker for isolated tumor cells in liver, lymph node, and bone marrow specimens of colorectal cancer patients. To detect these, a PCR-RFLP assay was used with a sensitivity exceeding

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