EZIL6

Human IL-6 ELISA Kit

detect IL-6 in 50 μL serum, plasma or cell culture supernatent

• Synonym(s): B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IL6, Interferon beta-2, Interleukin-6
• UNSPSC Code: 41116158
• NACRES: NA.77

Properties

• product name: Human IL-6 Conferma^® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples
• Quality Level: 200
• species reactivity: human
• parameter: 50 μL sample volume (4hr assay)
• assay range: linearity: 105-116%; (serum); linearity: 88-105%; (plasma); inter-assay cv: <15%; intra-assay cv: <5%; standard curve range: 1.17-300 pg/mL; accuracy: 91-109%
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma (K2 EDTA); sample type cell culture supernatant
• UniProt accession no.: P05231
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• Gene Information: human ... IL6(3569)

Description

General description

This Human Interleukin-6 (IL-6) Conferma^® ELISA kit is used for the quantification of Human IL-6 in serum, plasma, or culture media samples. Human IL-6 is a soluble protein cytokine most commonly associated with the acute inflammatory response to infection or tissue damage.

This is a 96 well format sandwich ELISA which uses our Conferma^® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs

Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.

UniProt Summary:

• Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
• Size: 212 amino acids; 23,718 Da
• Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
• Subcellular location: Extracellular region or secreted
• Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
• Similarity: Belongs to the IL-6 superfamily.

Application

One kit is sufficient to measure 37 samples in duplicate

Research Category:

• Cytokines
• Inflammation & Immunology
• Immune Response
• Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50 μL sample size (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.