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  • Effects of a sliding plate on morphology of the epiphyseal plate in goat distal femur.

Effects of a sliding plate on morphology of the epiphyseal plate in goat distal femur.

International journal of medical sciences (2012-02-24)
Da-sheng Lin, Ke-jian Lian, Jia-yuan Hong, Zhen-qi Ding, Wen-liang Zhai
ABSTRACT

The aim of this study was to observe the effects of a sliding plate on the morphology of the epiphyseal plate in goat distal femur. Eighteen premature female goats were divided randomly into sliding plate, regular plate and control groups. Radiographic analysis and histological staining were performed to evaluate the development of epiphyseal plate at 4 and 8 weeks after surgery. In the sliding plate group, the plate extended accordingly as the epiphyseal plate grows, and the epiphyseal morphology was kept essential normal. However, the phenomenon of the epiphyseal growth retardation and premature closure were very common in the regular plate group. In addition, the sliding plate group exhibited more normal histologic features and Safranin O staining compared to the regular plate group. Our results suggest that the sliding plate can provide reliable internal fixation of epiphyseal fracture without inhibiting epiphyseal growth.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gram′s safranin solution, for microscopy
Sigma-Aldrich
Safranin O, for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)
Sigma-Aldrich
Safranin O, Dye content ≥85 %
Sigma-Aldrich
Gram-Hucker′s stain solution (III)
Sigma-Aldrich
Safranin O, certified by the Biological Stain Commission