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  • Fluorescence in situ hybridization, a complementary molecular tool for the clinical diagnosis of infectious diseases by intracellular and fastidious bacteria.

Fluorescence in situ hybridization, a complementary molecular tool for the clinical diagnosis of infectious diseases by intracellular and fastidious bacteria.

FEMS microbiology reviews (2018-11-13)
Elsa Prudent, Didier Raoult
ABSTRACT

Many obligate or facultative intracellular bacteria pose a critical problem in clinical microbiology diagnosis as a result of their fastidious growth or lack of growth in conventional culture media. Molecular diagnosis is based on the analysis and demonstration of nucleic acids (DNA and RNA). In the field of infectiology, it combines laboratory medicine with the technology of molecular genetics to identify infectious pathogens. Fluorescence in situ hybridization (FISH) is used for the detection and localization of nucleotide sequences in various samples while preserving cell integrity. For more than 30 years, FISH methods have in constant evolution with the development of rRNA-targeted probes and synthetic molecules, such as PNA, which have contributed to the development of this technique in various fields by research and diagnostic laboratories. We describe here a panel of infectious diseases due to intracellular bacteria for which FISH diagnosis has proven its effectiveness. FISH techniques were applied in cases of blood-culture-negative endocarditis, respiratory infections, gastrointestinal diseases, mycobacterial infections, highly pathogenic microorganisms and other fastidious bacteria such as spirochetes. FISH has been proven to be applicable to various samples and for diverse infectious diseases, it can be used as a complementary tool for the diagnosis of infectious diseases by intracellular and fastidious bacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Eubacteria FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH), 20 µM in water
Sigma-Aldrich
Bacterial negative control FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Porphyromonas gingivalis FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Eubacteria FISH probe extension set, EUBII-Cy3 and EUBIII-Cy3 FISH (fluorescence in situ hybridization) probes for research, 20 μM in water
Sigma-Aldrich
Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water
Sigma-Aldrich
Akkermansia muciniphila FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water
Sigma-Aldrich
Escherichia coli FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)
Sigma-Aldrich
Bacterial negative control FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water
Sigma-Aldrich
Porphyromonas gingivalis FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH)