Human albumin has a molecular weight of approximately 67 kDa.
A9511
Albumin from human serum
lyophilized powder, ≥97% (agarose gel electrophoresis)
Synonym(s):
HSA
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About This Item
CAS Number:
UNSPSC Code:
12352202
eCl@ss:
42010201
EC Number:
274-272-6
NACRES:
NA.25
MDL number:
Biological source:
human
Assay:
≥97% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
ELISA: suitable, tissue culture: suitable, western blot: suitable
Impurities:
HIV I and HIVII, HCV and HBsAg, tested negative
Pricing and availability is not currently available.
biological source
human
Quality Segment
assay
≥97% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
monomer calculated mol wt 66478 Da
technique(s)
ELISA: suitable, tissue culture: suitable, western blot: suitable
impurities
HIV I and HIVII, HCV and HBsAg, tested negative
solubility
H2O: soluble 50 mg/mL
UniProt accession no.
storage temp.
2-8°C
SMILES string
FC(F)(F)C(F)(F)C(F)(F)F
InChI
1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11
InChI key
QYSGYZVSCZSLHT-UHFFFAOYSA-N
Gene Information
human ... ALB(213)
General description
Albumin is the most copious protein in blood plasma. Liver produces human albumin. [1]
Human albumin possess a molecular weight of 67 kDa.
Application
Albumin-bound fluorescence was used in serum of patients with chronic renal failure.[2]
Albumin from human serum has been used:
- to standardize the column for molecular-exclusion chromatography[2]
- to prepare the tobacco mosaic virus (TMV)-poly ethylene glycol (PEG8)-serum albumin (SA) particles
- to analyze its modifications after oxidation using a Fenton system
Biochem/physiol Actions
Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.
Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.
Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator in liver disease. Oxidative modification of albumin is seen in advanced liver disease.
Preparation Note
Prepared using method IV of Cohn, E.J., et al., J. Am. Chem. Soc., 69, 1753 (1947).
Other Notes
View more information on human serum albumin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human |
| assay ≥97% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) | assay 90-100% protein basis (biuret) | assay ≥90% (agarose gel electrophoresis) |
| technique(s) ELISA: suitable, western blot: suitable, tissue culture: suitable | technique(s) activity assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| impurities HIV I and HIVII, HCV and HBsAg, tested negative | impurities HIV I and HIVII, HCV and HBsAg, tested negative, ≤1.0 EU/mg endotoxin | impurities - | impurities - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the molecular weight of this product?
1 answer-
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アルブミンの屈折率 情報ありますか？
1 answer-
This product is not tested for refractive index, however, the incremental refractive index is reported in the general Human Serum Albumin datasheet.
See page 2, PHYSICAL PROPERTIES, at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/446/a9511pis.pdfHelpful?
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What are the impurities for Product A9511, Human serum albumin (HSA)?
1 answer-
The impurities present are alpha-globulin and beta-globulin.
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Can Product A9511, Human serum albumin (HSA), be used for cell culture applications?
1 answer-
Although A9511 is not sterile or endotoxin tested, it can probably still be used in cell culture. Filtering a solution of HSA through a 0.45 micron filter, then a 0.2 micron filter would provide a solution that is sterile, and likely suitable for cells. A5843 is an HSA product that has been endotoxin tested.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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