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CCYTOMAG-90K

Millipore

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonym(s):

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

species reactivity

canine

manufacturer/tradename

Milliplex®

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)

sensitivity: 21.0 pg/mL
(MCP-1)

sensitivity: 21.7 pg/mL
(IL-8)

sensitivity: 3.2 pg/mL
(IP-10)

sensitivity: 3.5 pg/mL
(IL-2)

sensitivity: 3.7 pg/mL
(IL-6)

sensitivity: 5.3 pg/mL
(KC-like)

sensitivity: 5.8 pg/mL
(IL-18)

sensitivity: 6.1 pg/mL
(TNFα)

sensitivity: 7.5 pg/mL
(IL-7)

sensitivity: 8.5 pg/mL
(IL-10)

sensitivity: 9.0 pg/mL
(IL-15)

sensitivity: 9.2 pg/mL
(GM-CSF)

standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
There was no or negligible cross-reactivity among different analytes within the panel.

Application

  • Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
  • Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
  • Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
  • Assay Run Time: Overnight at 4°C or 2 hours at room temperature (20-25° C)
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Linkage

Replaces: CCYTO-90K

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Bing Wang et al.
Acta biomaterialia, 116, 259-267 (2020-09-17)
In periodontal treatment, topical adjunctive therapy with antimicrobials or anti-inflammatory agents is frequently applied. However, currently available drug carrier biomaterials often exhibit poor perfusion into small crevices, such as the deep and irregular periodontal pockets, due to relatively high viscosity.
Chad B Maki et al.
Frontiers in veterinary science, 7, 570-570 (2020-10-29)
This study was conducted to investigate the therapeutic effect of allogeneic adipose-derived MSCs on dogs with hip osteoarthritis (OA). Twenty dogs with bilateral osteoarthritis of the coxofemoral (hip) joint, diagnosed by a veterinarian through physical examination and radiographs were randomly
Gustavo Rodrigues Martins et al.
Mediators of inflammation, 2016, 9512743-9512743 (2016-03-19)
Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between
Herman Borghys et al.
Journal of Alzheimer's disease : JAD, 56(2), 763-774 (2016-12-31)
Understanding differences in Alzheimer's disease biomarkers before the pathology becomes evident can contribute to an improved understanding of disease pathogenesis and treatment. A decrease in amyloid-β (Aβ)42 in cerebrospinal fluid (CSF) is suggested to be a biomarker for Aβ deposition
Anna Brunner et al.
Frontiers in veterinary science, 8, 652479-652479 (2021-07-06)
Background: The degree of systemic inflammation, reperfusion injury and endothelial activation are potentially important determinants of clinical outcomes in dogs with gastric dilatation volvulus (GDV). Objective: To evaluate plasma concentrations and kinetics of inflammatory markers in dogs with GDV over

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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