UHPLC Analysis of Dimethyl Sulfoxide and Dimethylformamide on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.0 μm particles (51406-U)

mobile phase

[A] 0.1% ammonium hydroxide; [B] acetonitrile; (5:95, A:B)

flow rate

0.4 mL/min

pressure

(205 bar)

column temp.

35 °C

detector

MS, ESI(+), MRM, 73.95/45.97, 78.85/63.92

injection

0.5 μL

sample

0.5 μg/mL each  in acetonitrile