RP-HPLC Analysis of Substituted Phenethylamine Psychedelics on Ascentis® Express 2.7 μm RP-Amide
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
25B-NBOMe hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®25I-NBOMe hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®25C-NBOMe hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®25I-NBF hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®25I-NBMD hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP-Amide 10cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase
[A] 20 mM Ammonium acetate pH 4.0 with Acetic Acid; [B] ACN; (85:15, A:B)
gradient
15% B for 2 min; 15 to 30% B in 6 min, held at 30% B for 4 min
flow rate
0.6 mL/min
pressure
4757 psi (328 bar)
column temp.
35 °C
detector
UV, 280 nm
injection
5 μL
sample
25C-NBOMe HCl, 25B-NBOMe HCl, 25I-NBF HCl, 25INBMD HCl, 25I-NBOME HCl, 20 μg/mL in 90:10 mobile phase A:mobile phase B
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express RP-Amide for the HPLC separation of substituted phenethylamine psychedelics (Bath Salts). This application shows the use of UV detection for this set of compounds.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany