RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Flunitrazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Lorazepam solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Nitrazepam solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Temazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Biphenyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (64066-U)
mobile phase
[A] Water; [B] acetonitrile; (70:30, A:B)
flow rate
1.0 mL/min
pressure
5294 psi (365 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
1.0 μL
sample
Benzodiazepine Multicomponent Mixture-8 diluted to 50ug/mL in 50:50 Water:Methanol
Description
Analysis Note
This analysis demonstrates the applicability of the Ascentis Express Biphenyl stationary phase for the analysis of benzodiazepines, a group of psychoactive drugs that act as GABA agonists. The stationary phase provides alternative selectivity and improved resolution to existing phenyl chemistires.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany